Surface Plasmon Resonance

Surface plasmon resonance P. Anton van der Merwe 1. 2. 2.1. origin PRINCIPLES AND APPLICATIONS OF SURFACE PLASMON RESONANCE. Principles 3 4 4 5 5 7 2.2. Applications 2.2.1. What SPR is entire for 2.2.2. What SPR is not good for. 3. 3.1. 3.2. 3.3. coarse PRINCIPLES OF BIACORE EXPERIMENTS A typical taste Preparation of materials and buffers Monitoring the Dips 8 8 9 9 4. 4.1. LIGAND Direct versus substantiative immobilization 10 10 11 11 12 12 12 14 15 16 16 17 17 18 19 19 4.2. covalent immobilisation 4.2.1. A worldwide approach 4.2.2. Choice of chemical science 4.2.3. Prepare the protein 4.2.4. Pre-concentration 4.2.5. Amine duad 4.2.6. Regeneration 4.2.7. Adjusting the immobilisation conditions. 4.3. Non-covalent immobilisation (ligand capture) 4.3.1. Using an existing system 4.3.2. Developing a bald-faced strategy 4.4. 4.5. 4.6. Activity of immobilised ligand hear surfaces Re-using sensor chips 5. 5.1. ANALYTE Purity, activity and concentration 20 20 -1- 5.2. 5.3. 5.4. valency Refractive index heart and soul and control analytes Low molecular(a) weight analytes 20 21 22 6. 6.1. 6.2. QUALITITATIVE ANALYSIS; DO THEY interact? Positive and negative controls qualitative comparisons using a multi-valued analyte 22 22 23 7.

QUANTITATIVE MEASUREMENTS 23 24 24 24 26 27 27 30 30 31 33 34 35 35 37 7.1. Affinity 7.1.1. Concepts 7.1.2. Experimental variant 7.1.3. info analysis 7.1.4. Controls 7.1.5. Non-linear Scatchard Plots 7.2. kinetics 7.2.1. Concepts 7.2.2. Experimental design 7.2.3. info analysis 7.2.4. Controls 7.3. 7.4. 7.5. Stoichiometry Thermodynamics Activation energy 8. 9. 9.1. finis APPENDIX Physical basis of SPR 38 38 38 39 39 40 41 9.2. Samples and buffers 9.2.1. Guidelines for preparing samples and buffers for use on the BIAcore 9.2.2. Standard buffers 9.3. Additional information 10. REFERENCES 11. ACKNOWLEDGEMENTS -2- 41 42 12. GLOSSARY 13. FIGURE 1....If you lot to get a broad essay, order it on our website: Orderessay

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